pU6-sgC2-VEGFA T1

LOCUS       pU6-sgC2-VEGFA T1               2307 bp DNA     circular SYN 20-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..2307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..262
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     rep_origin      complement(552..1140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1311..2171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2172..2276)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
       61 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      121 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      181 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      241 ttatatatct tgtggaaagg acgaaacacc ggtgggaggt ctgtccccac catgggcgtg
      301 cgaacctgct catcattgcg ttcgcacgtc caaacgacca tatcatcgtt gccctgcgac
      361 cattcagcgg caatcaagac gcaggcatga tatgtaacca tgcattggaa agtgcatgga
      421 gcagaagaaa cacagtagga gggacttttt tttttaagct tgggccgctc gaggtacctc
      481 tctacatatg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1201 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1261 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1321 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1381 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1441 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1501 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1561 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1621 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1681 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1741 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1801 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1861 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1921 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1981 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2041 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2101 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2161 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2221 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2281 tttccccgaa aagtgccacc tgacgtc
//